FIGURE 4 from Maternal Embryonic Leucine Zipper Kinase is Associated with Metastasis in Triple-negative Breast Cancer
Year: 2023
Type: other
Abstract: Effect of inhibition of MELK signaling on tumor growth and lung metastasis in TNBC xenograft mouse models. A, MELK expression in Cas9-p15 (P15) and MELK KO MDA-MB-231 (C3 and C28) cells. α-Tubulin was used as a loading control. B–F, MELK KO significantly suppressed lung metastases in an MDA-MB-231 xenograft mouse model. Female athymic nude mice were injected intravenously with luciferase-transfected Cas9-p15 or MELK KO MDA-MB-231-Luc-GFP stable cells. Metastatic tumors were measured weekly for 7 weeks by whole-body luciferase imaging using an IVIS 100 Imaging System. Shown are mouse whole-body luciferase images (B), photon counts per area (C), lung weight per mouse measured at week 7 following cell inoculation (D), mouse OS over a period of 80 days following cell inoculation (E), and IHC staining for hematoxylin and eosin (H&E), MELK, proliferation (Ki67), and mesenchymal markers (vimentin and fibronectin; F) in mice inoculated with Cas9-p15 or MELK KO MDA-MB-231 cells. Images were taken under 200 × magnification. G, MELK-In-17 significantly suppressed tumor growth in a 4T1 xenograft mouse model (P < 0.05). Murine 4T1 TNBC cells were injected into the mammary fat pads of female BALB/c mice. When tumor size reached 75–150 mm3, grouped mice were treated with vehicle or MELK-In-17 at 5 or 10 mg/kg via intraperitoneal injections daily for 25 days. In C–G, data are presented as mean ± SD. *, P < 0.05; **, P < 0.01.
Authors: Xuemei Xie, Gaurav B. Chauhan, Ramakrishna Edupuganti, Takahiro Kogawa, Jihyun Park, Moises Tacam, Alex W. Tan, Mohd Mughees, Fnu Vidhu, Diane D. Liu, Juliana M. Taliaferro, Mary Kathryn Pitner, Luke S. Browning, Ju-Hyeon Lee, François Bertucci, Yu Shen, Jian Wang, Naoto T. Ueno, Savitri Krishnamurthy, Gabriel N. Hortobagyi, Debu Tripathy, Steven J. Van Laere, Geoffrey Bartholomeusz, Kevin N. Dalby, Chandra Bartholomeusz
Language: English
Topic: Cancer Cells and Metastasis
Subfield: Oncology
Field: Medicine
Domain: Health Sciences
Open Access status: gold
OpenAlex ID: W4381382484