Systematic Transmission Electron Microscopy‐Based Identification and 3D Reconstruction of Cellular Degradation Machinery (Adv. Biology 6/2023)
Year: 2023
Type: article
Abstract: 3D Reconstruction Macroautophagy is a dynamic process that regulates cellular mechanisms. It can be difficult to identify machinery and quantify them. In article 2200221, Antentor O. Hinton Jr. and co-workers demonstrate the use of transmission electron microscopy, immunogold labeling, immunofluorescence, and 3D reconstruction serial block face-scanning electron microscopy to identify and quantify lysosomes, autophagosomes, and lipid droplets under drug response conditions and experimental knockouts.
Source: Advanced Biology
Authors: Kit Neikirk, Zer Vue, Prasanna Katti, Ben I. Rodriguez, Salem Omer, Jianqiang Shao, Trace Christensen, Edgar Garza Lopez, Andrea Marshall, Caroline B. Palavicino‐Maggio, Jessica Ponce, Ahmad F. Alghanem, Larry Vang, Taylor Barongan, Heather K. Beasley, Taylor Rodman, Dominique Stephens, Margaret Mungai, Marcelo Correia, Vernat Exil, Steven Damo, Sandra A. Murray, Amber Crabtree, Brian Glancy, Renata O. Pereira, E. Dale Abel, Antentor O. Hinton
Language: English
Subfield: Epidemiology
Field: Medicine
Domain: Health Sciences
Open Access status: bronze
OpenAlex ID: W4381887952