Year: 2023
Type: preprint
Abstract: Nanoscale fluorescence imaging with a large-field view is invaluable for many applications such as imaging of subcellular structures, visualizing protein interaction, and high-resolution tissue imaging. Unfortunately, conventional fluorescence microscopy has to make a trade-off between resolution and field of view due to the nature of the optics used to form an image. To overcome this barrier, we have developed an acoustofluidic scanning fluorescence nanoscope that can simultaneously achieve superior resolution, a large field of view, and enhanced fluorescent signal. The acoustofluidic scanning fluorescence nanoscope utilizes the super-resolution capability of microspheres that are controlled by a programable acoustofluidic device for rapid fluorescent enhancement and imaging. The acoustofluidic scanning fluorescence nanoscope can resolve structures that cannot be achieved with a conventional fluorescent microscope with the same objective lens and enhances the fluorescent signal by a factor of ~5 without altering the field of view of the image. The improved resolution with enhanced fluorescent signal and large field of view via the acoustofluidic scanning fluorescence nanoscope provides a powerful tool for versatile nanoscale fluorescence imaging for researchers in the fields of medicine, biology, biophysics, and biomedical engineering.
Source: Research Square
Institutions: University of Dayton, Duke University
Language: English
Subfield: Biomedical Engineering
Field: Engineering
Domain: Physical Sciences
Open Access status: green
Awards: G1034614284, Acoustofluidic Separation (AFS), Purification and Raman Spectral Fingerprinting of Single EVs: From Cell of Origin to Target Cell and Biofluids, Collaborative Research: Acoustic Holography Enabled Additive Manufacturing of High-resolution Multifunctional Composites, AFS/SERS Saliva-based SARS-CoV-2 Earliest Infection and Antibodies Detection, Three-Dimensional (3D) Acoustofluidic Scanning Nanoscope with Super Resolution and Large Field of View, Graduate Research Fellowship Program (GRFP), Acoustofluidic Separation of Placental Nanovesicle Subpopulations in Obstetrical Diseases
OpenAlex ID: W4382073184