Year: 2023
Type: article
Abstract: DNA-RNA hybrids play various roles in many physiological progresses, but how this chromatin structure is dynamically regulated during spermatogenesis remains largely unknown. Here, we show that germ cell-specific knockout of Rnaseh1, a specialized enzyme that degrades the RNA within DNA-RNA hybrids, impairs spermatogenesis and causes male infertility. Notably, Rnaseh1 knockout results in incomplete DNA repair and meiotic prophase I arrest. These defects arise from the altered RAD51 and DMC1 recruitment in zygotene spermatocytes. Furthermore, single-molecule experiments show that RNase H1 promotes recombinase recruitment to DNA by degrading RNA within DNA-RNA hybrids and allows nucleoprotein filaments formation. Overall, we uncover a function of RNase H1 in meiotic recombination, during which it processes DNA-RNA hybrids and facilitates recombinase recruitment.
Source: Nucleic Acids Research
DOI: 10.1093/nar/gkad524
Authors: Chao Liu, Liying Wang, Yanan Li, Mengmeng Guo, Jun Hu, Teng Wang, Mengjing Li, Zhuo Yang, Ruoyao Lin, Wei Xu, Yinghong Chen, Mengcheng Luo, Fei Gao, Jia-Yu Chen, Qianwen Sun, Hongbin Liu, Bo Sun, Wei Li
Institutions: Chinese Academy of Sciences, Guangzhou Women and Children Medical Center, Institute of Zoology, State Key Laboratory of Stem Cell and Reproductive Biology, Guangzhou Medical University, ShanghaiTech University, University of Chinese Academy of Sciences, State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Shandong University, Hospital for Reproductive Medicine Affiliated to Shandong University, Tsinghua University, Wuhan University, Center for Life Sciences
Language: English
Topic: DNA Repair Mechanisms
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $3,630
OpenAlex ID: W4382343943