Work

Electrical Impedance Determination of Cancer Cell Viability in a 3-Dimensional Cell Culture Microfluidic Chip

Year: 2012

Type: article

Abstract: A technique of quantitative determination of cell viability in three-dimensional (3D) cell culture construct is proposed based on on-site electrical impedance measurement in a microfluidic chip. In vitro 3D cell culture has been interpreted for faithfully representation of the in vivo cellular responses in living tissues. However, monitoring of the cellular responses in 3D cell culture construct is normally time-consuming and labor-intensive. In this work, a microfluidic chip was developed and is capable of on-site determining cell viability in 3D construct without affecting the cellular behaviors. The chip mainly consisted of a culture chamber, in which a pair of vertical electrodes at its opposite sidewalls was embedded for measurement, and a fluidic channel for perfusion purpose. Cancer cells/agarose suspension was loaded into the culture chamber for 3D cell culture with perfusing anti-cancer drug in different concentrations (6, 12, 18, and 24 μg ml-1) for 2 days. Since higher drug concentration led to more cell damage or death, the total impedance magnitude of the culture construct was shown to be reasonably proportional to the cell viability. The proposed technique has a high potential to develop a fast and easy measurement compared with the conventional cellular analysis techniques.

Source: International Journal of Electrochemical Science

DOI: 10.1016/s1452-3981(23)16588-6

Authors: Kin Fong Lei, Min-Hsien Wu, Che-Wei Hsu, Yi-Dao Chen

Institution: Chang Gung University

Language: English

Topic: Microfluidic and Bio-sensing Technologies

Subfield: Biomedical Engineering

Field: Engineering

Domain: Physical Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $1,670