Year: 2024
Type: article
Abstract: Light-sheet fluorescence microscopy (LSFM) in conjunction with tissue clearing techniques enables morphological investigation of large tissues faster and with excellent optical sectioning. Recently, cleared tissue axially swept light-sheet microscope (ctASLM) demonstrated three-dimensional isotropic resolution in millimeter-scaled tissues. But ASLM based microscopes suffer from low detection signal and slow imaging speed. Here we report a simple and efficient imaging platform that employs precise control of two fixed distant light-sheet foci to carry out ASLM. This allowed us to carry out full field of view (FOV) imaging at 40 frames per second (fps) which is a four-fold improvement compared to the current state-of-the-art. In addition, in a particular frame rate, our method doubles the signal compared to the current ASLM technique. To augment the overall imaging performance, we also developed a deep learning based tissue information classifier that enables faster determination of tissue boundary. We demonstrated the performance of our imaging platform on various cleared tissue samples and demonstrated its robustness over a wide range of clearing protocols.
Source: Communications Engineering
Language: English
Subfield: Biophysics
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $2,090
Funders: Nvidia, Takeda Science Foundation, Japan Agency for Medical Research and Development, Mochida Memorial Foundation for Medical and Pharmaceutical Research, Nakatani Foundation for Advancement of Measuring Technologies in Biomedical Engineering, National Institutes of Health, Department of Biology, University of New Mexico, Japan Society for the Promotion of Science, National Cancer Institute, National Institute of General Medical Sciences
Awards: 記憶メカニズムの多次元解析 - nmからメゾスケール/ミリ秒から日スケールまで, G6496325548, G7784204706, Creation of Neurophotonics and Elucidation of Brain Functions, G888082366, G9000029004, University of New Mexico Cancer Center Support Grant, Transformative lightsheet microscopy techniques for subcellular imaging in physiologically relevant 3D environments, Adaptive Tracking and Quantum Imaging for Protein-Protein Interactions
OpenAlex ID: W4393409366