FIGURE 2 from Immuno-PET Imaging of CD69 Visualizes T-Cell Activation and Predicts Survival Following Immunotherapy in Murine Glioblastoma
Year: 2023
Type: other
Abstract: Kinetics of activation markers on T cells following ICI treatment. Mice with intracranial GL261 gliomas were treated with ICI, and analyzed at the designated time points after treatment, as in Fig. 1. A, Percentages of CD69+ of CD8+ (left) and CD4+ TILs (right). B, Percentages of CD69+ of CD8+ (left) and CD4+ T cells (right) in spleen. C, Trajectories of CD69+/CD8+ in TILs (green line) and spleen (purple line) ratios at the designated time point compared with the pretreatment group. D, Percentages of PD-1+ of CD8+ (left) and CD4+ TILs (right). E, Percentages of PD-1+ of CD8+ (left) and CD4+ T cells (right) in spleen. F, Trajectories of PD-1+/CD8+ in TILs (blue line) and spleen (red line) ratios at the designated time point compared with the pretreatment group. G, Percentages of TIM3+ of CD69+/CD8+TILs. For A, B, D, E, and G, mean ± SEM values are shown. Scattered dots represent individual mice. Shown are representative data of two independent experiments. n = 4 per group. One-way ANOVA with multiple comparison test (*, P < 0.05; **, P <0.01; ***, P <0.001). C and F, Two-way ANOVA with multiple comparison test is used to compare the ratios between TILs and spleen T cells at each time point (*, P < 0.05; **, P <0.01; ***, P <0.001).
Authors: Michal Nisnboym, Sarah R. Vincze, Zujian Xiong, Chaim T. Sneiderman, Rebecca A. Raphael, Bo Li, Ambika P. Jaswal, ReidAnn E. Sever, Kathryn E. Day, Joseph D. LaToche, Lesley M. Foley, Hanieh Karimi, T. Kevin Hitchens, Sameer Agnihotri, Baoli Hu, Dhivyaa Rajasundaram, Carolyn J. Anderson, Deborah T. Blumenthal, Thomas M. Pearce, Shikhar Uttam, Jessie R. Nedrow, Ashok Panigrahy, Ian F. Pollack, Frank S. Lieberman, Jan Drappatz, Itay Raphael, Wilson B. Edwards, Gary Kohanbash
Language: English
Subfield: Oncology
Field: Medicine
Domain: Health Sciences
Open Access status: gold
OpenAlex ID: W4383341550