Figure 4 from A Compendium of Syngeneic, Transplantable Pediatric High-Grade Glioma Models Reveals Subtype-Specific Therapeutic Vulnerabilities
Year: 2023
Type: other
Abstract: A, Mutation combinations represented in H3K27M models and the acronyms used to refer to them. B, IHC detection of hematoxylin and eosin (H&E), GFP, K27M, and Ki-67 in coronal hindbrain sections from tumor-bearing, symptomatic mice. Embryos were electroporated with vectors encoding (i) H3.3K27M, p53LOF (KP); (ii) H3.1K27M, p53LOF (H3.1KP); (iii) H3.3K27M, FGFR1N457K (KF); (iv) H3.3K27M, NF1LOF (KN); (v) H3.3K27M, p53LOF, FGFR1N457K (KPF); and (vi) H3.3K27M, p53LOF, CCND2WT (KPC). Tumor cells are GFP+. Higher magnification panels below each low-magnification panel are of the tumor bulk in the brainstem, i.e., pons and medulla, which are directly below the cerebellum and fourth ventricle. Scale bars, 2 mm in the low-magnification panels and 200 μm in the high-magnification panels. C, Kaplan–Meier survival curves of IUE, tumor-bearing mice carrying different combinations of mutations. KP (n = 6), H3.1KP (n = 6), KF (n = 10), KN (n = 7), KPF (n = 16), and KPC (n = 9). Statistical comparisons using the log-rank Mantel–Cox tests are described in Supplementary Table S2.
Language: English
Subfield: Genetics
Field: Medicine
Domain: Health Sciences
Open Access status: gold
OpenAlex ID: W4383535789