Figure 5 from A Compendium of Syngeneic, Transplantable Pediatric High-Grade Glioma Models Reveals Subtype-Specific Therapeutic Vulnerabilities
Year: 2023
Type: other
Abstract: A, Top: penetrance of spinal dissemination in tumor-bearing mice harboring H3.3K27M, NF1LOF, and FGFR1N457K mutations compared with all other genotypes. Bottom: representative bright field (BF) and fluorescence (GFP) images of GFP+ tumor cells that have disseminated into the thoracic spinal cord. Scale bars, 1 mm. B, Top: penetrance of exophytic spread in tumor-bearing mice harboring FGFR1N457K, PIK3CAE545K, PPM1DΔC, and NF1LOF. Bottom: representative BF and fluorescence images showing exophytic spread (bottom left) or a lack of exophytic spread (bottom right) of GFP+ tumor cells. Scale bars, 1 mm. C, Top: penetrance of cranial nerve invasion in tumor-bearing KF, KPP, H3.1KACVPIK, and KPPMPIK mice. Bottom: representative BF and fluorescence images showing cranial nerve invasion of GFP+ tumor cells (indicated by white arrows). Scale bars, 1 mm. D, Analysis of the extent of invasion of H3.1/3K27M-driven tumors into different anatomic regions (CB, cerebellum; BS, brainstem). E, Immunofluorescence and quantification of Ki-67+/GFP+ cells in H3.1/3K27M- and H3.3G34R-driven tumors. GFP+ tumor cells have a mitotic index of 10 or higher. Data, mean ± SEM. Representative images showing DAPI, GFP, and Ki-67 immunofluorescence levels in a brainstem tumor are shown on the right. Scale bars, 50 μm. F, Quantification of H3K27me3 immunofluorescence signal intensity in HA+ nuclei in tumors. Data, mean ± SEM; ****, P < 0.0001 was calculated using the one-way ANOVA test. Representative images of H3K27me3 and HA staining in a brainstem tumor are shown on the right. Scale bars, 50 μm. a.u., arbitrary units.
Language: English
Subfield: Genetics
Field: Medicine
Domain: Health Sciences
Open Access status: gold
OpenAlex ID: W4383553654