Circular RNA hsa_circ_001350 contributes to osteosarcoma progression by regulating microRNA-578/CCR4-NOT transcription complex and subunit 7/Wnt signaling.
Year: 2023
Type: article
Abstract: Accumulating evidence has revealed that circular RNAs (circRNAs) play important roles in cancer by sponging microRNAs (miRNAs). A previous study has shown that hsa_circ_001350 expression is increased in glioma tissue samples and cells and that hsa_circ_001350 directly sponges miR-1236. Here, we investigated the role of hsa_circ_001350 in osteosarcoma (OS). Bioinformatics analysis was performed to examine the potential interactions among hsa_circ_001350, miR-578, and the CCR4-NOT transcription complex and subunit 7 (CNOT7). Reverse transcription-quantitative polymerase chain reaction and western blotting were performed to analyze gene expression and protein levels, respectively. Hsa_circ_001350 expression was upregulated in OS tissues and cell lines. The deletion of hsa_circ_001350 inhibited the proliferation, migration, and invasion of OS cells. The downregulation of hsa_circ_001350 suppressed CNOT7 expression by sponging miR-578 as confirmed by rescue experiments and luciferase reporter assays. Specifically, the depletion of hsa_circ_001350 inhibited the protein expression of β-catenin, cyclin D1, and c-myc in OS cells, and CNOT7 overexpression reversed this effect. We conclude that hsa_circ_001350 contributes to OS progression by regulating miR-578/CNOT7/Wnt signaling. Thus, hsa_circ_001350, miR-578, and CNOT7 may be potential targets for the treatment of OS.
Source: PubMed Central
Authors: Rujin Xu, Xingyuan Ci, Fang He, Yueqin Chen
Language: English
Topic: Circular RNAs in diseases
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W4383710382