FIGURE 1 from The African-centric P47S Variant of TP53 Confers Immune Dysregulation and Impaired Response to Immune Checkpoint Inhibition
Year: 2023
Type: other
Abstract: The tumor growth advantage in S47 mice is abolished following CD8+ T-cell depletion. A, Illustration of the experimental approach. Replicate P47 and S47 mice were injected subcutaneously with 1 × 106 MC38 tumor cells. At day 19, spleens and tumors from mice were harvested and analyzed by flow cytometry. B, Tumor growth curves for P47 and S47 mice injected with MC38 cells. n = 5 mice per group. Linear mixed model used for statistical analysis. **, P < 0.01. C, Tumor weights of MC38 tumors harvested from P47 and S47 mice. n = 5 mice per group. Wilcoxon rank-sum test used for statistical analysis. *, P < 0.05. D and E, Tumor growth curves for P47 and S47 mice injected with MC38 cells, ± treatment with anti-CD8b antibody. n = 5 mice per group. Linear mixed model used for statistical analysis. *, P < 0.05. F, The percentage of CD8+ T cells of total T cells (CD45+/CD3+) in P47 and S47 mice before and after CD8+ T-cell depletion is shown on the left. Shown on the right are the total counts of splenic CD8+ T cells in P47 and S47 mice before and after CD8+ T-cell depletion.
Language: English
Subfield: Oncology
Field: Medicine
Domain: Health Sciences
Open Access status: gold
OpenAlex ID: W4383982486