The Versatile Biocatalyst of Cytochrome P450 CYP102A1: Structure, Function, and Engineering
Year: 2023
Type: review
Abstract: is a highly efficient monooxygenase for the oxidation of long-chain fatty acids. The unique features of CYP102A1, such as high catalytic activity, expression yield, regio- and stereoselectivity, and self-sufficiency in electron transfer as a fusion protein, afford the requirements for an ideal biocatalyst. In the past three decades, remarkable progress has been made in engineering CYP102A1 for applications in drug discovery, biosynthesis, and biotechnology. The repertoire of engineered CYP102A1 variants has grown tremendously, whereas the substrate repertoire is avalanched to encompass alkanes, alkenes, aromatics, organic solvents, pharmaceuticals, drugs, and many more. In this article, we highlight the major advances in the past five years in our understanding of the structure and function of CYP102A1 and the methodologies used to engineer CYP102A1 for novel applications. The objective is to provide a succinct review of the latest developments with reference to the body of CYP102A1-related literature.
Source: Molecules
Institutions: University of Michigan, Michigan Medicine
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $2,558
Awards: Development of gene editing based therapy for cardiovascular diseases, IDOL and dyslipidemia in cardiovascular diseases, Structural basis for detoxification of environmental pollutants by native complexes of CYP2B family with its redox partners, P450 and NO Synthase Regulation by Multiprotein Complexes, Structure-based computational engineering of saCas9 PAM requirement, G8783077706, Browning of perivascular adipose tissue protects against thoracic aortic aneurysm
OpenAlex ID: W4384203069