Work

Linker, loading, and reaction scale influence automated glycan assembly

Year: 2023

Type: article

Abstract: Automated glycan assembly (AGA) affords collections of well-defined glycans in a short amount of time. We systematically analyzed how parameters connected to the solid support affect the AGA outcome for three different glycan sequences. We showed that, while loading and reaction scale did not significantly influence the AGA outcome, the chemical nature of the linker dramatically altered the isolated yields. We identified that the major determinants of AGA yields are cleavage from the solid support and post-AGA purification steps.

Source: Beilstein Journal of Organic Chemistry

DOI: 10.3762/bjoc.19.77

Authors: Marlene C S Dal Colle, Manuel G Ricardo, Nives Hribernik, José Danglad-Flores, Peter H Seeberger, Martina Delbianco

Institutions: Max Planck Institute of Colloids and Interfaces, Freie Universität Berlin

Language: English

Topic: Carbohydrate Chemistry and Synthesis

Subfield: Organic Chemistry

Field: Chemistry

Domain: Physical Sciences

Open Access status: diamond

Funders: Deutsche Forschungsgemeinschaft, Bundesministerium für Bildung und Forschung, Max-Planck-Gesellschaft