Year: 2024
Type: article
Abstract: Wound healing is impaired by infection; however, how microbe-induced inflammation modulates tissue repair remains unclear. We took advantage of the optical transparency of zebrafish and a genetically tractable microbe, Listeria monocytogenes , to probe the role of infection and inflammation in wound healing. Infection with bacteria engineered to activate the inflammasome, Lm-Pyro, induced persistent inflammation and impaired healing despite low bacterial burden. Inflammatory infections induced il1b expression and blocking IL-1R signaling partially rescued wound healing in the presence of persistent infection. We found a critical window of microbial clearance necessary to limit persistent inflammation and enable efficient wound repair. Taken together, our findings suggest that the dynamics of microbe-induced tissue inflammation impacts repair in complex tissue damage independent of bacterial load, with a critical early window for efficient tissue repair.
Source: iScience
Institution: University of Wisconsin–Madison
Language: English
Topic: Wound Healing and Treatments
Subfield: Rehabilitation
Field: Medicine
Domain: Health Sciences
Open Access status: gold
Estimated APC paid: $3,150
Awards: Identification of Listeria monocytogenes immune evasion mechanisms, Mechanisms of macrophage-dependent neutrophil reverse migration during tissue damage, G5610402250, Metabolic regulation of macrophage-dependent wound healing in vivo, Identification and Characterization of Genes Required for Listeria monocytogenes Cytosolic Survival, Metabolic regulation of macrophage-dependent wound healing in vivo, Cell migration and wound repair
OpenAlex ID: W4392967100