Year: 2023
Type: conference-paper
Abstract: In the field of Structural Biology, Atom Probe Tomography (APT) is in the nascent stages of development wherein coarse-grained visuals of proteins have been captured. However, no aspect of protein structure has been determined. The characterization of organic samples or biomolecules through the technique is currently limited to the detection of a few dominant signatures. The problem of indecipherable characterization can inherently be traced back to multiple forms of technique-specific responses to organic samples and consequent triggers leading to organic-sample and sample-medium interactions. As a first counter to the characterization problem, by taking the experiment-specific uncertainties, and probable APT-centric organic sample-based variabilities into account, the current results of the study are presented. Through mass-spectrometric and computational analysis, specific individual amino acids (Sulfur-containing protein-bound amino acids) in proteins and aspects of protein structure (probable backbone fragments) have been identified and characterized. Additionally, to overcome technique-specific deterrents to the characterization of biomolecules in cryogenic sample medium, the development of a protein-labeling strategy tailored to APT is suggested.
DOI: 10.2172/1986492
Institution: Pacific Northwest National Laboratory
Language: English
Subfield: General Materials Science
Field: Materials Science
Domain: Physical Sciences
Open Access status: green
OpenAlex ID: W4384643749