Work

Aptamer-Based Chromatographic Methods for Efficient and Economical Separation of Leukocyte Populations

Year: 2023

Type: article

Abstract: The manufacturing process of chimeric antigen receptor T cell therapies includes isolation systems that provide pure T cells. Current magnetic-activated cell sorting and immunoaffinity chromatography methods produce desired cells with high purity and yield but require expensive equipment and reagents and involve time-consuming incubation steps. Here, we demonstrate that aptamers can be employed in a continuous-flow resin platform for both depletion of monocytes and selection of CD8 + T cells from peripheral blood mononuclear cells at low cost with high purity and throughput. Aptamer-mediated cell selection could potentially enable fully synthetic, traceless isolations of leukocyte subsets from a single isolation system.

Source: ACS Biomaterials Science & Engineering

DOI: 10.1021/acsbiomaterials.3c00651