Work

Base-editing screens illuminate variant effects in human hematopoiesis

Year: 2023

Type: article

Abstract: , Martin-Rufino et al. develop a strategy for performing high-throughput base-editing CRISPR screens coupled with single-cell readouts in the context of human hematopoiesis. Through a series of proof-of-principle experiments, the authors demonstrate the potential of base-editing screens for the study and treatment of hematological disorders.

Source: Cell Reports Methods

DOI: 10.1016/j.crmeth.2023.100541

Authors: Alina Vaitsiankova, Tanay Thakar, Alberto Ciccia

Institutions: Columbia University Irving Medical Center, Herbert Irving Comprehensive Cancer Center

Language: English

Topic: CRISPR and Genetic Engineering

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $5,200