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FIGURE 3 from Clinically Defined Mutations in MEN1 Alter Its Tumor-suppressive Function Through Increased Menin Turnover

Year: 2023

Type: other

Abstract: R516fs and E235K mutations exhibit reduced protein stability and shortened half-lives compared with wild-type (WT) menin. A, Western blot analysis of MEFΔMen1 whole-cell extracts following overexpression of wild-type menin in the presence of the protein synthesis inhibitor cycloheximide (CHX, 10 μmol/L) alone or pretreated with the proteosome inhibitor MG132 (10 μmol/L). B, Normalized FLAG band signal intensity plotted as a function of time in the presence of CHX with and without MG132 pretreatment. Overexpression of the R516fs (C–D), E235K(E, F), and A541T (G, H) menin proteins with normalized FLAG band signal intensity plotted as a function of time in the presence of CHX alone or in combination with MG132 pretreatment. n = 3 experimental replicates, mean ± SEM. *, P < 0.05; ***, P < 0.01; ****, P < 0.0001 by two-way ANOVA.

DOI: 10.1158/2767-9764.23736808.v1

Authors: Suzann Duan, Sulaiman Sheriff, Uloma B. Elvis-Offiah, Brandon L. Witten, Travis W. Sawyer, Sinju Sundaresan, Tomasz Cierpicki, Jolanta Grembecka, Juanita L. Merchant

Language: English

Topic: Neuroendocrine Tumor Research Advances

Subfield: Epidemiology

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: gold

OpenAlex ID: W4385198587

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