FIGURE 2 from Proteogenomic Approaches for the Identification of NF1/Neurofibromin-depleted Estrogen Receptor–positive Breast Cancers for Targeted Treatment
Year: 2023
Type: other
Abstract: Detection of NF1 by a MS-based approach (KIPA) after pulldown by beads conjugated to kinase inhibitors. A,NF1 mRNA was determined by RNA-seq, while NF1 protein levels (iBAQ, the sum of all the peptides intensities divided by the number of theoretical peptides from the protein) was quantified by KIPA. Correlation coefficient and P value were determined by Pearson correlation. NF1 copy-number status was as described in Fig. 1. B, Protein correlation with NF1 protein kinase levels as determined by KIPA in PDXs. P values and R were determined by a linear regression model. C, Volcano plot shows differences in protein abundance as log2FC (fold change) in baseline POL samples that were stratified into NF1-low versus NF1-high groups. The heat map shows relative levels of indicated proteins. P values determined by Student t test.
Authors: Beom-Jun Kim, Ze-Yi Zheng, Jonathan T. Lei, Matthew V. Holt, Anran Chen, Jianheng Peng, Diana Fandino, Purba Singh, Hilda Kennedy, Yongchao Dou, María del Rosario Chica-Parrado, Emmanuel Bikorimana, Dan Ye, Yunguan Wang, Ariella B. Hanker, Nada Mohamed, Susan G. Hilsenbeck, Bora Lim, Jaya Ruth Asirvatham, Arun Sreekumar, Bing Zhang, George Miles, Meenakshi Anurag, Matthew J. Ellis, Eric C. Chang
Language: English
Subfield: Cancer Research
Domain: Life Sciences
Open Access status: gold
OpenAlex ID: W4385284976