Work

Computational refocusing in phase-resolved confocal microscopy

Year: 2023

Type: article

Abstract: We demonstrate numerical refocusing in coherent confocal laser scanning microscopy based on synthetic optical holography. In this physics-based approach, computational propagation is implemented on the complex signal recovered in synthetic holography, consistent with wave physics and the parameters of the microscope. An experimental demonstration is shown to restore an in-focus image of a test object from data acquired at several focal plane off-sets. Numerical refocusing can provide focused views on samples with large height variation, with a potential application in confocal optical surface profiling.

Source: Optics Letters

DOI: 10.1364/ol.496111

Authors: Martin Schnell, Melanie King, Sam Buercklin, Paulo Sarriugarte, Rainer Hillenbrand, P. Scott Carney

Institutions: Ikerbasque, University of Illinois Urbana-Champaign, University of Rochester, University of the Basque Country

Language: English

Topic: Digital Holography and Microscopy

Subfield: Atomic and Molecular Physics, and Optics

Field: Physics and Astronomy

Domain: Physical Sciences

Open Access status: gold

Funder: Ministerio de Ciencia e Innovación

Awards: G210248140, G773377789