P27-017-23 Curcumin Attenuates Obesity Induced Hypertension in Male but Not Female Mice
Year: 2023
Type: article
Abstract: Objectives: Maternal one-carbon metabolism, including dietary levels of folic acid and choline, play an important role in early life programming.There is a well-established connection between the fetal environment and the health status of offspring.However, there is a gap in knowledge on how maternal nutrition will affect the health status of the offspring after a cardiovascular event like ischemic stroke.The aim of our study was to investigate the role of maternal dietary deficiencies in folic acid or choline on stroke outcome in 3-and 11-month-old male and female offspring.Methods: Adult female mice were fed a folic acid deficient diet (FADD), a choline deficient diet (ChDD), or a control diet (CD) four weeks prior to pregnancy to deplete stores, they were continued on diets during pregnancy and lactation.Male and female offspring were weaned onto a control diet and at 2 or 10 months of age were subject to ischemic stroke within the sensorimotor cortex via the photothrombosis ischemic damage model.At 3 or 11 months of age, motor function was measured in offspring and tissue was collected for analysis.Results: Mothers maintained on either a FADD or ChDD had reduced levels of S-adenosylmethionine in liver tissue compared to controls.In offspring after ischemic stroke, motor function was impaired in 3-month-old male and female offspring from deficient mothers compared to control diet offspring.In 11month-old mice there was no impact of maternal diet on motor function, but we observed sex differences.Male middle-aged adult mice had worse motor function compared to female offspring.In brain tissue, there was no impact of maternal diet on ischemic damage volume in 3-month-old animals.Interestingly, maternal diet impacted 10-month-old male and female offspring.Neurodegeneration and choline metabolism in ischemic brain tissue was impacted in both 3 and 11-month-old offspring.Conclusions: The findings of our study suggest that a maternal diet deficient in either choline or folic acid impacts stroke outcome in young animals compared to middle-aged animals.This result points to the important role of the maternal diet in early life programming, while emphasizing its effects on both fetal development and long-term cerebrovascular health.
Authors: Kevin John, Maria Barnes
Language: English
Field: Medicine
Domain: Health Sciences
Open Access status: gold
Estimated APC paid: $4,000
OpenAlex ID: W4385368103