Work

Two-photon manipulation of neuronal activity and behavior in Hydra vulgaris

Year: 2023

Type: article

Abstract: The introduction of calcium imaging has rendered cnidarians, such as Hydra vulgaris, valuable model organisms for investigating neuronal activity and behavior. Here, we present a comprehensive protocol to image and manipulate neuronal activity and behavior of Hydra. We describe steps for wide-field imaging and two-photon simulation and ablation of neurons. We then detail imaging behavior and post-ablation analysis. We address challenges that may arise during the preparation and execution of the experiments.

Source: STAR Protocols

DOI: 10.1016/j.xpro.2023.102453

Authors: Wataru Yamamoto, Rafael Yuste

Institution: Columbia University

Language: English

Topic: Marine Invertebrate Physiology and Ecology

Subfield: Paleontology

Field: Earth and Planetary Sciences

Domain: Physical Sciences

Open Access status: gold

Estimated APC paid: $1,500

Funders: National Science Foundation, U.S. Department of Defense, Office of Naval Research