Protocol to assess receptor-ligand binding in C. elegans using adapted thermal shift assays
Year: 2023
Type: article
Abstract: The Caenorhabditis elegans genome encodes a greatly expanded number of nuclear hormone receptors, many of which remain orphaned. Here, we present a protocol to assess ligand-receptor binding in C. elegans using an adapted cellular thermal shift assay and isothermal dose response. We describe steps for growing C. elegans and preparing lysates and compounds. We also detail how to perform and quantify these assays. This protocol can be used to study any soluble receptor. For complete details on the use and execution of this protocol, please refer to Peterson et al. (2023).1
Source: STAR Protocols
Authors: Samantha Y. Tse, Read Pukkila-Worley
Institution: University of Massachusetts Chan Medical School
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $1,500
Awards: Neuroendocrine regulation of intestinal epithelial immunity in C. elegans, Medical Scientist Training at UMMS, Detection of intestinal pathogens through host surveillance of bacterial toxins, Innate Immunity Training Program, An Immune Surveillance Network in C. elegans, Detection of pathogen infection by monitoring host cell membrane dynamics
OpenAlex ID: W4385436927