Figure 2 from β-Catenin Drives Butyrophilin-like Molecule Loss and γδ T-cell Exclusion in Colon Cancer
Year: 2023
Type: other
Abstract: γδ T cells are excluded from mouse and human gut tumors. A, Images of SI tissue from 4 WT (Cre-negative), tumor-bearing Villin-CreERT2;ApcF/+ (VA) and tumor-bearing Villin-CreERT2;ApcF/+;KrasG12D (VAK) mice stained for Trdc mRNA; scale bar, 500 μm. B, γδ T-cell numbers in SI tissue of indicated models. Each dot represents one mouse (n = 11 WT, 3 VA, 3 VAK). C, Representative flow cytometry plot of CD8α and γδTCR expressions on total CD3+ cells in the small intestine of WT mice. CD8α+ γδ T-cell frequency in SI of indicated models. Each dot represents one mouse (n = 9 WT, 4 VA, 5 VAK). D, Images of SI from indicated models (n = 4/group) stained for Trdc mRNA; scale bar, 500 μm. E, γδ T-cell numbers in SI of indicated models. Each dot represents one mouse (n = 3 WT, 4 VAF/F, 4 VAF/FK). F, Image of γδ T-cell staining in tumor adjacent tissue and tumor tissue from human colon cancer sections (Scotland cohort, n = 141) where arrows indicate positively stained cells; scale bar, 500 μm. G, Density of γδ T cells in human colon cancer sections in three different patient cohorts: Scotland (n = 141), Norway (n = 71), and Thailand (n = 122). γδ T cells identified by IHC in full sections were quantified in tumor adjacent tissue or tumor tissue using Visiopharm. Data are presented as median ± min/max. H, Expressions of TRGV4 and TRGV9 mRNA in human colon cancer samples (n = 82) from the Scotland cohort determined by TempO-Seq. Data are presented as mean ± SD. *, P < 0.05; **, P < 0.01; ***, P < 0.001 (paired t test or one-way ANOVA followed by Dunnett post hoc test).
Authors: Toshiyasu Suzuki, Anna Kilbey, Nuria Casa-Rodríguez, Amy Lawlor, Anastasia Georgakopoulou, Hannah Hayman, Kyi Lai Yin Swe, Anna Nordin, Claudio Cantù, Pierre Vantourout, Rachel A. Ridgway, Ryan M. Byrne, Lei Chen, Michael P. Verzi, David M. Gay, Ester Gil Vázquez, Hayley L. Belnoue-Davis, Kathryn Gilroy, Anne Helene Køstner, Christian Kersten, Chanitra Thuwajit, Ditte K. Andersen, Robert Wiesheu, Anett Jandke, Karen Blyth, Antonia K. Roseweir, Simon J. Leedham, Philip D. Dunne, Joanne Edwards, Adrian Hayday, Owen J. Sansom, Seth B. Coffelt
Language: English
Subfield: Oncology
Field: Medicine
Domain: Health Sciences
Open Access status: gold
OpenAlex ID: W4385549622