Figure 6 from β-Catenin Drives Butyrophilin-like Molecule Loss and γδ T-cell Exclusion in Colon Cancer
Year: 2023
Type: other
Abstract: Activation of β-catenin decreases Hnf4a and Hnf4g expressions. A, Expressions of HNF4A and HNF4G in normal human colonic tissue and tumor tissue from TCGA (n = 19 normal, 101 tumor) and Skrypczak (n = 24 normal, 45 tumor) datasets. Data are presented as median ± minimum/maximum. *, P < 0.05 (Mann–Whitney U test). B,Hnf4a and Hnf4g expressions are determined by RNA-seq analysis of SI in WT (n = 9), VAF/F (n = 36), and VAF/FK (n = 17) mice. Each dot represents one mouse. C, Images of HNF4A and HNF4G protein expressions in SI of indicated models (n = 4); scale bar, 500 μm. D, Images of intestinal tissue from tumor-bearing VA mice stained for indicated proteins; scale bar, 500 μm. E, Fold change in expression levels of Hnf4a and Hnf4g in organoids from various genotypes measured at indicated days after tamoxifen treatment. Each dot represents one organoid from one mouse (n = 3). F, Fold change in expression levels of Hnf4a and Hnf4g in WT organoids treated with 3 or 10 μmol/L CHIR-99021 for indicated days. Each dot represents one organoid from one mouse (n = 3). Data are presented as mean ± SD. **, P < 0.01; ***, P < 0.001 (one-way ANOVA followed by Dunnett post hoc test).
Authors: Toshiyasu Suzuki, Anna Kilbey, Nuria Casa-Rodríguez, Amy Lawlor, Anastasia Georgakopoulou, Hannah Hayman, Kyi Lai Yin Swe, Anna Nordin, Claudio Cantù, Pierre Vantourout, Rachel A. Ridgway, Ryan M. Byrne, Lei Chen, Michael P. Verzi, David M. Gay, Ester Gil Vázquez, Hayley L. Belnoue-Davis, Kathryn Gilroy, Anne Helene Køstner, Christian Kersten, Chanitra Thuwajit, Ditte K. Andersen, Robert Wiesheu, Anett Jandke, Karen Blyth, Antonia K. Roseweir, Simon J. Leedham, Philip D. Dunne, Joanne Edwards, Adrian Hayday, Owen J. Sansom, Seth B. Coffelt
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
OpenAlex ID: W4385549628