Figure 3 from β-Catenin Drives Butyrophilin-like Molecule Loss and γδ T-cell Exclusion in Colon Cancer
Year: 2023
Type: other
Abstract: Expression of butyrophilin-like molecules is reduced in gut tumors. A, Images of intestinal tissue from indicated models (n = 4) stained for Btnl1 mRNA. T, tumor; scale bar, 100 μm. B, Images of intestinal tissue from indicated models (n = 4) stained for Btnl1 mRNA; scale bar, 100 μm. C, Butyrophilin-like mRNA expression shown by bar graph generated from RNA-seq data from WT (n = 9), VAF/F (n = 36), and VAF/FK (n = 17) mice. Data are presented as mean ± SD. D, Expressions of BTNL3 and BTNL8 in normal human colonic tissue and tumor tissue from TCGA (n = 19 normal, 101 tumor; ref. 9) and Skrypczak (n = 24 normal, 45 tumor; ref. 27) datasets. Data are presented as median ± min/max. E–G, Correlation between indicated molecules as determined by TempO-Seq and γδ T-cell density determined by IHC in the Scotland cohort from 77 matched pairs. Units on axes are normalized read counts x 103. Each dot represents one tumor. P and r values determined by Pearson correlation. *, P < 0.05; **, P < 0.01; ***, P < 0.001 (Mann–Whitney U test or one-way ANOVA followed by Tukey post hoc test).
Authors: Toshiyasu Suzuki, Anna Kilbey, Nuria Casa-Rodríguez, Amy Lawlor, Anastasia Georgakopoulou, Hannah Hayman, Kyi Lai Yin Swe, Anna Nordin, Claudio Cantù, Pierre Vantourout, Rachel A. Ridgway, Ryan M. Byrne, Lei Chen, Michael P. Verzi, David M. Gay, Ester Gil Vázquez, Hayley L. Belnoue-Davis, Kathryn Gilroy, Anne Helene Køstner, Christian Kersten, Chanitra Thuwajit, Ditte K. Andersen, Robert Wiesheu, Anett Jandke, Karen Blyth, Antonia K. Roseweir, Simon J. Leedham, Philip D. Dunne, Joanne Edwards, Adrian Hayday, Owen J. Sansom, Seth B. Coffelt
Language: English
Subfield: Oncology
Field: Medicine
Domain: Health Sciences
Open Access status: gold
OpenAlex ID: W4385549487