Massively parallel reporter assay confirms regulatory potential of hQTLs and reveals important variants in lupus and other autoimmune diseases
Year: 2024
Type: article
Abstract: Objective: To systematically characterize the potential for histone post-translational modifications, i.e., histone quantitative trait loci (hQTLs), expression QTLs (eQTLs), and variants on systemic lupus erythematosus (SLE) and autoimmune (AI) disease risk haplotypes to modulate gene expression in an allele dependent manner. Methods: We designed a massively parallel reporter assay (MPRA) containing ~32K variants and transfected it into an Epstein-Barr virus transformed B cell line generated from an SLE case. Results: Our study expands our understanding of hQTLs, illustrating that epigenetic QTLs are more likely to contribute to functional mechanisms than eQTLs and other variant types, and a large proportion of hQTLs overlap transcription start sites (TSS) of noncoding RNAs. In addition, we nominate 17 variants (including 11 novel) as putative causal variants for SLE and another 14 for various other AI diseases, prioritizing these variants for future functional studies primary and immortalized B cells. Conclusion: We uncover important insights into the mechanistic relationships between genotype, epigenetics, gene expression, and SLE and AI disease phenotypes.
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $3,300
Funders: Presbyterian Health Foundation, Cincinnati Children's Hospital Medical Center, Oklahoma Medical Research Foundation, National Institutes of Health, University at Buffalo, National Institute of General Medical Sciences, National Institute of Allergy and Infectious Diseases, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Center for Research Resources, National Center for Advancing Translational Sciences
Awards: Science in a Culture of Mentoring, Molecular Mechanisms and Genetics of Autoimmunity, G4360243898, G4527139674, G4582235923, Oklahoma Rheumatic Disease Research Cores Center, Epigenome-Guided Causal Variant Discovery and Mechanisms, G9000007649, G9000320916, G9000335128, Disease and Race Specific Single-cell Epigenetic Mechanisms in Human SLE
OpenAlex ID: W4392112311