Work

Connexin 43 and cell culture substrate differentially regulate OCY454 osteocytic differentiation and signaling to primary bone cells

Year: 2023

Type: article

Abstract: 3D cell culture of OCY454 cells promoted increased differentiation compared with traditional 2D culture. Although Cx43 deficiency did not affect OCY454 differentiation, it resulted in increased signaling, promoting osteoblastogenesis and osteoclastogenesis. Our results suggest that Cx43 deficiency promotes increased bone remodeling in a cell-autonomous manner with minimal changes in osteocyte differentiation. Also, 3D cultures appear better suited to study mechanisms in Cx43-deficient OCY454 osteocytes.

Source: American Journal of Physiology-Cell Physiology

DOI: 10.1152/ajpcell.00220.2023

Authors: Gabriel A. Hoppock, Evan G. Buettmann, Joseph A. Denisco, Galen M Goldscheitter, Sebastian N. Condyles, Otto J. Juhl, Michael A. Friedman, Yue Zhang, Henry J. Donahue

Institution: Virginia Commonwealth University

Language: English

Topic: Bone Metabolism and Diseases

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: green

Funders: National Aeronautics and Space Administration, Lowy Medical Research Institute, Nuclear Safety and Security Commission, National Institutes of Health, National Cancer Institute, National Institute of Arthritis and Musculoskeletal and Skin Diseases

Awards: G1354076642, G2524726931, G3947550534, THIS PROPOSAL FOR A TRANSLATIONAL RESEARCH INSTITUTE IS SUBMITTED ON BEHALF OF A CONSORTIUM OF NON-PROFIT ACADEMIC INSTITUTIONS IN RESPONSE TO NASA C, G6027020843, G9000186613, G9000279430, Massey Cancer Center - Cancer Center Support Grant

OpenAlex ID: W4386020248

FWCI: 0.819

Cites: 45

Cited by: 11

Related to: 10