Supplementary Materials and Methods from High-Throughput Drug Screening of Primary Tumor Cells Identifies Therapeutic Strategies for Treating Children with High-Risk Cancer
Year: 2023
Type: supplementary-materials
Abstract: Supplementary Materials and Methods Establishment of patient-derived xenograft modelsAnimal studies were approved by the Animal Care and Ethics Committee of UNSW Sydney, approval numbers 17/101B, 19/82B and 20/82B.Patient-derived xenograft (PDX) models were generated by intravenous inoculation of mononuclear cells from the bone marrow (for leukemia) or subcutaneous implantation of tumor pieces from biopsies (for other cancers) in NOD/SCID/IL-2 receptor gamma-/-(NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJAusb; NSG) mice as described previously (1,2).Tumor burden was measured once a week by flow cytometry analysis of the percentages of human CD45-positive cells (for leukemia) or twice a week by digital caliper measurements of tumor sizes (for other cancers).When having reached >80% CD45-positive cells or a tumor size of 1,000 mm 3 , tumors (cells) were collected and immediately processed for HTS, cryopreserved till further use for HTS or
Authors: Chelsea Mayoh, Jie Mao, Jinhan Xie, Gabor Tax, Shu-Oi Chow, Roxanne Cadiz, Karina Pazaky, Paulette Barahona, Pamela Ajuyah, Peter Trebilcock, Angela Malquori, Kate Gunther, Anica Avila, Doo Young Yun, Stephanie Alfred, Anjana Gopalakrishnan, Alvin Kamili, Marie Wong, Mark J. Cowley, Sophie Jessop, Loretta M.S. Lau, Toby N. Trahair, David S. Ziegler, Jamie I. Fletcher, Andrew J. Gifford, Maria Tsoli, Glenn M. Marshall, Michelle Haber, Vanessa Tyrrell, Timothy W. Failes, Greg M. Arndt, Richard B. Lock, Paul G. Ekert, M. Emmy M. Dolman
Language: English
Subfield: Cancer Research
Domain: Life Sciences
Open Access status: gold
OpenAlex ID: W4386026209