FIGURE 7 from Natural Killer Cell Activation by Ubiquitin-specific Protease 6 Mediates Tumor Suppression in Ewing Sarcoma
Year: 2023
Type: other
Abstract: USP6 elicits an abscopal response with infiltration and activation of NK cells in DTs. A, Experimental set-up to test abscopal response using bilateral subcutaneous xenografts (n = 10 per cohort). B, Volume of DTs in the absence or presence of dox. Tumors were digested, and subjected to flow cytometry to quantify: expression levels of NKp46 in the NK1.1+/F4/80−/Ly6G− NK cell population in PTs and DTs (C); activation, proliferative capacity, and NKG2D expression in NKs in DTs (D); and activation of DCs and macrophages in PTs and DTs, as monitored by MHCII levels (n = 8 for –dox, and n = 10 for +dox; E). F, Summary and model of immunostimulatory and antitumorigenic functions of USP6 in Ewing sarcoma. Left, mechanism of NK cell activation and paracrine immunostimulatory feedforward loop; right, depiction of abscopal response (diagram created using Biorender.com).
Language: English
Subfield: Immunology
Field: Immunology and Microbiology
Domain: Life Sciences
Open Access status: gold
OpenAlex ID: W4386067968