Year: 2023
Type: article
Abstract: Comparing brain structure across species and regions enables key functional insights. Leveraging publicly available data from a novel mass cytometry-based method, synaptometry by time of flight (SynTOF), we applied an unsupervised machine learning approach to conduct a comparative study of presynapse molecular abundance across three species and three brain regions. We used neural networks and their attractive properties to model complex relationships among high dimensional data to develop a unified, unsupervised framework for comparing the profile of more than 4.5 million single presynapses among normal human, macaque, and mouse samples. An extensive validation showed the feasibility of performing cross-species comparison using SynTOF profiling. Integrative analysis of the abundance of 20 presynaptic proteins revealed near-complete separation between primates and mice involving synaptic pruning, cellular energy, lipid metabolism, and neurotransmission. In addition, our analysis revealed a strong overlap between the presynaptic composition of human and macaque in the cerebral cortex and neostriatum. Our unique approach illuminates species- and region-specific variation in presynapse molecular composition.
Source: Scientific Reports
Authors: Eloïse Berson, Chandresh R. Gajera, Thanaphong Phongpreecha, Amalia Perna, Syed A. Bukhari, Martin Becker, Alan L. Chang, Davide De Francesco, Camilo Espinosa, Neal G. Ravindra, Nadia Postupna, Caitlin S. Latimer, Carol A. Shively, Thomas C. Register, Suzanne Craft, Kathleen S. Montine, Edward J. Fox, C. Dirk Keene, Sean C. Bendall, Nima Aghaeepour, Thomas J. Montine
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $2,390
Awards: Social Stress, Diet. and Primate Monocyte Programming in Cardiovascular Risk, Machine Learning for Integrative Modeling of the Immune System in Clinical Settings, Wake Forest Alzheimer's Disease Core Center, The Phenotypic Landscape of Cognitive Decline as Revealed by Next-Generation Multiplexed Ion Beam Imaging, G3798839418, Dietary Mitigation of Psychosocial Stress Effects on CVD Risk , Neuropathologic substrates for motor and cognitive impairment in three existing cohort studies of Alzheimer's disease and related dementias, BD Canto II RUO Cytometer, Neuropathology of synapses in AD and ADRD, MIRIAD - Multiplexed Imaging of Resilience In Alzheimers Disease, Effects of Western and Mediterranean Diets on Metabolic and Neuropathologic Risk Factors for Alzheimer's Disease in Nonhuman Primates, G9000013408, G9000087139, Deciphering the Glycan Code in Human Alzheimer's Disease Brain, Adult Changes in Thought (ACT) Research Program, University of Washington Alzheimer's Disease Research Center, Alzheimer's Disease Research Center, Uncoupling Age- Versus Cognitive-Related Cellular Senescence in Alzheimer's Disease
OpenAlex ID: W4386120985