Work

Biomolecular interactions studied by low-field NMR using SABRE hyperpolarization

Year: 2023

Type: article

Abstract: compared to Boltzmann polarization at the measurement field indicates that this experiment is not feasible without prepolarization. The extended magnetic field range for the measurement of biomolecular interactions under near physiological conditions, with a protein concentration on the order of 10 μM or less, provides a new option for screening of ligand binding, measurement of protein-protein interactions, and measurement of molecular dynamics.

Source: Chemical Science

DOI: 10.1039/d3sc02365f

Authors: Pierce Pham, Christian Hilty

Institution: Texas A&M University

Language: English

Topic: Advanced NMR Techniques and Applications

Subfield: Spectroscopy

Field: Chemistry

Domain: Physical Sciences

Open Access status: diamond

Funders: National Science Foundation, Welch Foundation, Division of Chemistry

Awards: G1020944575, G2988109435, G6089863091, Characterization of Macromolecular Dynamics Using Para-hydrogen Induced Polarization of Nuclear Spins