Integrated antigenic and nucleic acid detection in single virions and virion-infected host-derived extracellular vesicles
Year: 2023
Type: preprint
Abstract: Virion-mediated outbreaks are imminent and despite rapid responses, they continue to cause adverse symptoms and death. Therefore, tunable, sensitive, high-throughput assays are needed to control future virion-mediated outbreaks. Herein, we developed a tunable in situ assay to selectively sort virions and infected host-derived extracellular vesicles (IHD-EVs) and simultaneously detect antigens and nucleic acids at a single-particle resolution. The Biochip Antigen and RNA Assay (BARA) enhanced sensitivities, enabling the detection of virions in asymptomatic patients, genetic mutations in single virions, and the continued long-term expression of virion-RNA in the IHD-EVs of post-acute sequelae of COVID-19 patients. The BARA revealed highly accurate diagnoses by simultaneously detecting the spike glycoprotein and nucleocapsid-encoding RNA on single SARS-CoV-2 virions in saliva and nasopharyngeal swab samples. Altogether, the single-particle detection of antigens and virion-RNA provides a tunable framework for the diagnosis, monitoring, and mutation screening of current and future outbreaks. Teaser The BARA enables antigenic and nucleic acid testing in single virions for unprecedented perspectives on viral diseases
Source: medRxiv
Authors: Kim Truc Nguyen, Xilal Y. Rima, Luong T. H. Nguyen, Xinyu Wang, Kwang Joo Kwak, Min Jin Yoon, Hong Li, Chi-Ling Chiang, Jacob Doon-Ralls, Kelsey Scherler, Shannon Fallen, Stephanie L. Godfrey, Julie A. Wallick, Setty M. Magaña, Andre F. Palmer, Inyoul Lee, Christopher C. Nunn, Kimberly M. Reeves, Henry G. Kaplan, Jason D. Goldman, James R. Heath, Kai Wang, Preeti Pancholi, L. James Lee, Eduardo Reátegui
Institutions: The Ohio State University, The Ohio State University Wexner Medical Center, Institute for Systems Biology, Swedish Medical Center, Nationwide Children's Hospital, University of Washington, The Ohio State University Comprehensive Cancer Center – Arthur G. James Cancer Hospital and Richard J. Solove Research Institute
Language: English
Subfield: Infectious Diseases
Field: Medicine
Domain: Health Sciences
Open Access status: green
OpenAlex ID: W4386400828