The genetic diversity and genetic structure of the populations of Campylopus umbellatus (Arn.) Paris based on ISSR
Year: 2019
Type: article
Abstract: To study the genetic diversity and population genetic structure characteristics of Campylopus umbellatus(Arn.) Paris,inter-simple sequence repeat (ISSR) marker technique method was used in the current study.40 individuals of 8 populations collected from Zhejiang,Jiangsu,Guangxi,Yunnan and Taiwan were detected by 12 ISSR primers.These primers generated 172 clear,repeatable bands.After analyzed by POPGENE 3.2 software,it was found that there were 158 polymorphic loci,of which the percentage of polymorphic bands(PPB) was 91.86%,Nei's Genetic Diversity Index (H) was 0.2922,Shannon-Wiener Index (I) was 0.4459.The results indicated that the populations of Campylopus umbellatus has high level of genetic diversity.The genetic differentiation index (Gst) of the eight populations was 0.6346,the gene flow (Nm) among populations was 0.2879,and the majority of genetic variation (63.33%) exists among populations,only 36.67% exists in populations,which showed that Campylopus umbellatus has obvious genetic differentiation among populations.Based on ISSR data,the dendrogram with a grouping threshold at a genetic distance of 65 showed that populations could be divided into 6 groups.According to our analyses,the genetic differentiation among the populations of Campylopus umbellatus mainly is caused by geographical factors,which may related to the habitat heterogeneity.
Authors: SHAN Yiyi, SHEN Lin, LI Dandan, YU Jing
Institution: Shanghai Normal University
Language: English
Subfield: Genetics
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W4386436594