Antibiotic prophylaxis dysregulates dental implant placement surgery‐induced osteoimmune wound healing and attenuates the alveolar bone–implant interface in mice
Year: 2023
Type: article
Abstract: Aim Antimicrobial‐induced shifts in commensal oral microbiota can dysregulate helper T‐cell oral immunity to affect osteoclast–osteoblast actions in alveolar bone. Antibiotic prophylaxis is commonly performed with dental implant placement surgery to prevent post‐surgical complications. However, antibiotic prophylaxis effects on osteoimmune processes supporting dental implant osseointegration are unknown. The aim of the study was to discern the impact of antibiotic prophylaxis on dental implant placement surgery‐induced osteoimmune wound healing and osseointegration. Materials and Methods We performed SHAM or dental implant placement surgery in mice. Groups were administered prophylactic antibiotics (amoxicillin or clindamycin) or vehicle. Gingival bacteriome was assessed via 16S sequencing. Helper T‐cell oral immunity was evaluated by flow cytometry. Osteoclasts and osteoblasts were assessed via histomorphometry. Implant osseointegration was evaluated by micro‐computed tomography. Results Dental implant placement surgery up‐regulated TH1, TH2 and TREG cells in cervical lymph nodes (CLNs), which infers helper T‐cell oral immunity contributes to dental implant placement osseous wound healing. Prophylactic antibiotics with dental implant placement surgery caused a bacterial dysbiosis, suppressed TH1, TH2 and TREG cells in CLNs, reduced osteoclasts and osteoblasts lining peri‐implant alveolar bone, and attenuated the alveolar bone–implant interface. Conclusions Antibiotic prophylaxis dysregulates dental implant placement surgery‐induced osteoimmune wound healing and attenuates the alveolar bone–implant interface in mice.
DOI: 10.1111/jcpe.13875
Authors: Waqar Ahmad, Novin Pishevar, Leonard J. Cochrane, Andrew Reynolds, Joseph Kim, Ivan Korostenskij, Vincenza L. Geiser, Matthew D. Carson, Amy J. Warner, Peng Chen, Hai Yao, Alexander Alekseyenko, Jessica D. Hathaway‐Schrader, Chad M. Novince
Institutions: Medical University of South Carolina, Clemson University
Language: English
Subfield: Oral Surgery
Field: Dentistry
Domain: Health Sciences
Open Access status: bronze
Funders: U.S. Department of Health and Human Services, Medical University of South Carolina, American Society for Bone and Mineral Research, National Institutes of Health, National Institute on Aging, National Institute of General Medical Sciences, National Institute of Diabetes and Digestive and Kidney Diseases, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institute of Dental and Craniofacial Research
Awards: Age-Related Kynurenine Accumulation Impairs miRNA and Hdac Epigenetic Regulation of the SDF-1 Axis Resulting in Bone Loss, G2808578740, Impact of Tetracycline Antibiotics on Skeletal Maturation, Mechanistic probes to study the immune response in periodontal disease, Impact of the Microbiome on Osteoimmunology and Skeletal Development, SC COBRE for TranslationalResearch Improving MusculoskeletalHealth (SC-TRIMH), T-COHR: Training in Craniofacial and Oral Health Research, Integrating craniofacial morphology, oral function, temporomandibular biomechanics and mechanobiology to determine sex-specific TMJ pathophysiology in humans, COBRE in Digestive and Liver Disease, MUSC Digestive Disease Research Core Center
OpenAlex ID: W4386439138