Figure 2 from Upregulation of Somatostatin Receptor Type 2 Improves 177Lu-DOTATATE Therapy in Receptor-Deficient Pancreatic Neuroendocrine Tumor Model
Year: 2023
Type: other
Abstract: HDACis increase SSTR2 transcription and protein expression in three NET cell lines. A, BON-1 cells treated with VPA at 2 mmol/L for 48 hours (P = 0.0001) and 72 hours (P = 0.0144) significantly increased SSTR2 mRNA expression. B, BON-1 cells treated with CI-994 at 2.5 μmol/L for 48 hours (P = 0.0001) and 72 hours (P = 0.03), and at 5 μmol/L for 48 hours (P = 0.0001) and 72 hours (P = 0.0001) had a significant increase in SSTR2 expression. C, QGP-1 cells treated with VPA at 2 mmol/L for 72 hours had a significant increase in SSTR2 expression (P = 0.0379). D, QGP-1 cells treated with CI-994 at 5 μmol/L for 48 hours (P = 0.0392) had a significant increase in SSTR2 expression. E, GOT-1 cells treated with CI-994 at 2.5 μmol/L for 48 hours (P = 0.0008) and 72 hours (P = 0.0022) showed a significant increase in SSTR2 expression. F, HDACis VPA and CI-994 increased SSTR2 protein expression in BON-1 cells 1.4- and 2.8-fold, respectively, with a concomitant increase in H3-histone acetylation at 48 hours. G, HDACis VPA and CI-994 increased SSTR2 protein expression in QGP-1 cells 2.18- and 3.48-fold, respectively, with a concomitant increase in histone acetylation at 48 hours. H, Graphical representation of the FC in total SSTR2 protein expression in BON-1 and QGP-1 cells. Data are expressed as mean ± SEM, (n = 3). *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001.
Language: English
Subfield: Epidemiology
Field: Medicine
Domain: Health Sciences
Open Access status: gold
OpenAlex ID: W4386475080