FIGURE 3 from Single-cell Profiling Uncovers a Muc4-Expressing Metaplastic Gastric Cell Type Sustained by Helicobacter pylori-driven Inflammation
Year: 2023
Type: other
Abstract: Gastric immunosuppression prevents metaplastic pit cell expansion. A–G, Flow cytometry was performed at 6 weeks using Hp−KRAS+ and Hp+KRAS− mice, and Hp+KRAS+ mice who received either oral dexamethasone (“dex”) starting at 2 weeks or water alone (control, “ctrl”). Graphs indicate the number of live leukocytes isolated from the gastric lamina propria of the indicated mouse groups. CD4−CD8−Ly6C+Ly6G+ neutrophils (A), CD11b variable Ly6C+ MHC II– circulating monocytes (B), CD64+F4/80+ macrophages (C), CD11c high MHC II high conventional dendritic cells (D), CD3+CD8α+CD103+CD44+ effector T cells (E), CD3+CD4+CD103+CD44+ effector T cells (F), CD3+CD4+CD103+CD44+FoxP3+ regulatory T cells (G). N = 2 independent experiments were conducted and data from one experiment is shown. Datapoints represent actual values for each individual mouse, zeroes are plotted at the limit of detection (10 cells), and bars indicate median values. H–J,Hp+KRAS+ mice that were control-treated or dexamethasone-treated are shown. H,Hp was cultured from stomach homogenate supernatants at time of euthanasia. CFU, colony-forming units. Zeroes are plotted at the limit of detection (10 CFU). I and J,Muc4 (brown) was detected by ISH in N = 2 independent experiments with n = 3–6 mice per group. I, Muc4 expression was scored in a blinded fashion. J, Representative images are shown. Scale bars, 100 μm. †, P < 0.10; *, P ≤ 0.05; **, P < 0.01; ns, not significant, Mann–Whitney U test.
Language: English
Subfield: Surgery
Field: Medicine
Domain: Health Sciences
Open Access status: gold
OpenAlex ID: W4386485688