FIGURE 3 from A Druggable FOXA1-Glucocorticoid Receptor Transcriptional Axis Drives Tumor Growth in a Subset of Non-Small Cell Lung Cancer
Year: 2023
Type: other
Abstract: FOXA1 and GR regulate the expression of targets involved in growth factor receptor activation and cell-cycle progression. A, Venn diagram depicting overlap in FOXA1 binding events following vehicle or dexamethasone stimulation and GR binding events following dexamethasone stimulation in H441 cells. B, GSEA of 789 genes bearing FOXA1 and GR overlapping binding events. C, IGV browser views for GR ChIP-seq (black) and FOXA1 ChIP-seq (pink) in H441 cells cultured in full serum media and treated with vehicle or 100 nmol/L dexamethasone for 30 minutes as indicated. Tracks shown are combined peaks from two independent replicates. D, ChIP-qPCR analysis of GR, FOXA1, and IgG following 30 minutes of indicated treatment. Rabbit isotype IgG was used as a negative control. Values correspond to mean percentage of input chromatin ± SEM of duplicate qPCR reactions. E, Motif analysis at FOXA1 and GR binding regions in indicated treatment conditions. F, Immunoblots of FOXA1, GR, and β-Actin in H441 cells expressing inducible shRNAs targeting FOXA1, GR, or FOXA1 and GR, treated with 1 μg/mL doxycycline for 48 hours. G, mRNA levels of FOXA1, NR3C1 (GR), and FOXA1/GR targets following treatment with 1 μg/mL doxycycline for 72 hours. Fold change in mRNA is normalized to 0 hour. CT values for each cell line. Values correspond to mean of three replicates ± SD.
Language: English
Subfield: Genetics
Domain: Life Sciences
Open Access status: gold
OpenAlex ID: W4386498362