Work

A computer vision based optical method for measuring fluid level in cell culture plates

Year: 2023

Type: article

Abstract: For a transparent well with a known volume capacity, changes in fluid level result in predictable changes in magnification of an overhead light source. For a given well size and fluid, the relationship between volume and magnification can be calculated if the fluid's index of refraction is known or in a naive fashion with a calibration procedure. Light source magnification can be measured through a camera and processed using computer vision contour analysis with OpenCV. This principle was applied in the design of a 3D printable sensing device using a raspberry pi zero and a camera.

Source: PLoS ONE

DOI: 10.1371/journal.pone.0290951

Authors: Pierre V. Baudin, Mircea Teodorescu

Institution: University of California, Santa Cruz

Language: English

Topic: Optical measurement and interference techniques

Subfield: Computer Vision and Pattern Recognition

Field: Computer Science

Domain: Physical Sciences

Open Access status: gold

Estimated APC paid: $1,805

Funders: Schmidt Futures, National Science Foundation, National Human Genome Research Institute