Work

A method for in vivo evaluation of α-glucosidase inhibition using Drosophila

Year: 2023

Type: article

Abstract: The development of α-glucosidase inhibitors is essential for the prevention of type II diabetes. Previous research has investigated in vitro inhibition using isolated α-glucosidase, which may not accurately reflect physical processes. The method presented in this study aims to establish a rapid and inexpensive in vivo method to study the inhibition of α-glucosidase activity using Drosophila as a model organism. This method can be used to calculate the IC 50 value of compounds of interest for inhibition of α-glucosidase activity. The method established in this study can be used for in vivo screening of anti-diabetic compounds. •A rapid and inexpensive in vivo method to study the inhibition of α-glucosidase activity. •This method can be used to calculate the IC 50 value of compounds of interest for inhibition of α-glucosidase activity. •This is a useful method for in vivo screening of anti-diabetic compounds.

Source: MethodsX

DOI: 10.1016/j.mex.2023.102373

Authors: Nattapong Wongchum, Alongklod Tanomtong, Somchai Pinlaor, Chatmongkon Suwannapoom, Ananya Dechakhamphu

Institutions: Ubon Ratchathani Rajabhat University, Khon Kaen University, University of Phayao

Language: English

Topic: Natural Antidiabetic Agents Studies

Subfield: Endocrinology, Diabetes and Metabolism

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $530