Year: 2023
Type: article
Abstract: . Mechanistically, we identified miR146a-5p as a sponge miRNA of circCRIM1 through bioinformatic prediction and confirmed their interaction and colocalization via reporter gene assay and FISH analysis. This interaction leads to increase expression of the downstream target gene NUMB, which will cause inhibition of the Notch signal pathway. We further demonstrated that miR146a-5p overexpression could reverse the antitumor effect induced by circCRIM1 in OS cells. Our results support that circCRIM1 acts as a tumor suppressor in OS by sponging miR146a-5p and its downstream target NUMB.
Source: PubMed Central
Authors: Jinnan Chen, Ruoxuan Hei, Chen Chen, Xuan Wu, Tiaotiao Han, Huiqin Bian, Junxia Gu, Yaojuan Lu, Qiping Zheng
Institutions: Jiangsu University, Tang Du Hospital, Air Force Medical University
Language: English
Topic: Circular RNAs in diseases
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
Award: G3225605126
OpenAlex ID: W4386591667