In Vitro Tri-culture Blood-brain Barrier (bbb) Model Enabling Direct Intercellular Contact at a Suspended Layer
Year: 2022
Type: conference-paper
Abstract: This paper reports the development of an in vitro tri-culture BBB model that forms suspended BBB layers where direct intercellular connections among BBB cells are established.The in vitro BBB model consists of top and bottom culture chambers and a porous membrane with 30 µm holes, whose hole diameter is 2~3 times larger than the size of cells to allow direct cell-to-cell communication.The porous membrane was coated with hydrogel and a mixture of collagen IV and fibronectin, and the three types of BBB cells of the human brain microvascular endothelial cells, pericytes, and astrocytes were cultured on the membrane.The establishment of the tri-cultured BBB layers was validated by immunostaining.Immunostaining images provided a clear expression of three different identification proteins of the BBB cells confirming the establishment.Then, the successful culture of cells on the large-hole membrane was validated by the measurement of high TEER values of 67 Ω•cm -2 and low permeability coefficient values of 2.9×10 -7 cm•s -1 .In summary, the development of the in vitro tri-culture BBB model was successfully delivered.
Institution: University of Utah
Language: English
Subfield: Neurology
Field: Neuroscience
Domain: Life Sciences
Open Access status: bronze
OpenAlex ID: W4386823462