Cell-type-specific control of secondary cell wall formation by Musashi-type translational regulators in Arabidopsis
Year: 2023
Type: article
Abstract: Deciphering the mechanism of secondary cell wall/SCW formation in plants is key to understanding their development and the molecular basis of biomass recalcitrance. Although transcriptional regulation is essential for SCW formation, little is known about the implication of post-transcriptional mechanisms in this process. Here we report that two bonafide RNA-binding proteins homologous to the animal translational regulator Musashi, MSIL2 and MSIL4, function redundantly to control SCW formation in Arabidopsis. MSIL2/4 interactomes are similar and enriched in proteins involved in mRNA binding and translational regulation. MSIL2/4 mutations alter SCW formation in the fibers, leading to a reduction in lignin deposition, and an increase of 4-O-glucuronoxylan methylation. In accordance, quantitative proteomics of stems reveal an overaccumulation of glucuronoxylan biosynthetic machinery, including GXM3, in the msil2/4 mutant stem. We showed that MSIL4 immunoprecipitates GXM mRNAs, suggesting a novel aspect of SCW regulation, linking post-transcriptional control to the regulation of SCW biosynthesis genes.
Source: eLife
Authors: Alicia Kairouani, Dominique Pontier, Claire Picart, Fabien Mounet, Yves Martinez, Lucie Le-Bot, Mathieu Fanuel, Philippe Hammann, Lucid Belmudes, Rémy Merret, Jacinthe Azevedo, Marie-Christine Carpentier, Dominique Gagliardi, Yohann Couté, Richard Sibout, Natacha Bies-Etheve, Thierry Lagrange
Institutions: Centre National de la Recherche Scientifique, Université de Perpignan, Laboratoire Génome et Développement des Plantes, Université Toulouse III - Paul Sabatier, Laboratoire de Recherche en Sciences Végétales, Université de Toulouse, Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement, Biopolymères Interactions Assemblages, Université de Strasbourg, Inserm, Commissariat à l'Énergie Atomique et aux Énergies Alternatives, CEA Grenoble, Université Grenoble Alpes, Institut de Biologie Moléculaire des Plantes
Language: English
Subfield: Plant Science
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $2,500
Awards: Infrastructure Française de Protéomique, G1502922282, CBH-EUR-GS, Functional analysis of a new siRNA-dependent transcriptional gene silencing pathway that targets rapidly evolving genomic regions of the plant genome., Par-delà les frontières, l'Université de Strasbourg, G2255650664, G2659019386, G2694206344, Façonner les talents en formation et en recherche à l'Université de Strasbourg, G2980570810, G3357951378, G3730343, G399734812, G4373231291, Integrative Molecular and Cellular Biology, G4656594441, G471331628, G4779185514, The Argonaute hook motif: its function in RNA silencing in Arabidopsis and viral/bacterial mechanisms of evasion in plants., G6136357505, G6803667113, G7240651115, The Toulouse-Perpignan
OpenAlex ID: W4380482492