FIGURE 6 from Pancreatic Ductal Adenocarcinoma Cells Regulate NLRP3 Activation to Generate a Tolerogenic Microenvironment
Year: 2023
Type: other
Abstract: CSF-1R engagement induces IL1β and IL18 release. A, Correlation between CSF-1R and NLRP3 expression in PDAC (n = 96) from TGCA dataset. B, Spontaneous CSF-1 and IL34 secretion in Panc-1 cells at 72 hours (n = 3). C, IL1β and IL18 secretion in THP-1 cells following stimulation with PDAC-conditioned media obtained from Panc-1 cells in presence of IgG (control), α-CSF-1, α-IL34, α-CSF-1/IL34 (n = 3). D, Phosphorylation levels of PyK2 (Tyrosine 402) in THP-1 stimulated in D and E (n = 3). E, IL1β and IL18 secretion from THP-1 cells following stimulation with PDAC-conditioned media obtained from Panc-1 cells in presence of α-CSF-1R and an CSF-1R inhibitor (AZD; n = 3). F, IL1β secretion from freshly isolated PBMCs from healthy donors (n = 4) following stimulation with PDAC-conditioned media obtained from Panc-1 cells in presence of α-CSF-1R. Cytokine levels were measured by specific ELISAs. Data expressed as mean ± SEM; ****, P < 0.0001; ***, P < 0.001; **, P < 0.01; *, P < 0.05.
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
OpenAlex ID: W4386889487