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FIGURE 2 from Pancreatic Ductal Adenocarcinoma Cells Regulate NLRP3 Activation to Generate a Tolerogenic Microenvironment

Year: 2023
Type: other
Abstract: PDAC paracrine activity mediates NLRP3 activation. A, IL1β and IL18 secretion from freshly isolated PBMCs from healthy donors (n = 6) following stimulation with PDAC-conditioned media obtained from AsPC-1, MiaPaCa-2, and Panc-1 cells. B, IL1β and IL18 secretion from parenteral (WT) and NLRP3 deficient (NLRP3−/−) THP-1 cells following stimulation with PDAC-conditioned media obtained from Panc-1 cells (n = 3). C, IL1β and IL18 secretion from THP-1 cells following stimulation with PDAC-conditioned media obtained from Panc-1 cells in presence of the NLRP3 inhibitors OLT1177 (OLT) and MCC950 (MCC; n = 3). Cytokine levels were measured by specific ELISA kits. Data expressed as mean ± SEM; ****, P < 0.0001; **, P < 0.01; *, P < 0.05.
DOI: 10.1158/2767-9764.24168346
Authors: Jesus Amo-Aparicio, Adrian Dominguez, Shaikh M. Atif, Alberto Dinarello, Tania Azam, Kibrom M. Alula, Miles Piper, Christopher H. Lieu, Robert W. Lentz, Alexis D. Leal, Stacey M. Bagby, Wells A. Messersmith, Sana D. Karam, Charles A. Dinarello, Todd M. Pitts, Carlo Marchetti
Language: English
Open Access status: gold
OpenAlex ID: W4386896974
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