Work

Protocol for screening facultative parthenogenesis in Drosophila

Year: 2023

Type: article

Abstract: Most species of sexually reproducing Drosophila are capable of some degree of facultative parthenogenesis, which involves the initiation of development in an unfertilized egg. Here, we present an optimized protocol to screen facultative parthenogenesis in Drosophila. We describe steps for the collection and maintenance of virgin flies. We then detail offspring screening for the analysis of parthenogenesis. This protocol can be applied to different Drosophila strains and can be adapted for the analysis of parthenogenesis in other animals. For complete details on the use and execution of this protocol, please refer to Sperling et al.1

Source: STAR Protocols

DOI: 10.1016/j.xpro.2023.102585

Authors: Alexis L. Sperling, David M. Glover

Institutions: University of Cambridge, California Institute of Technology

Language: English

Topic: Animal Behavior and Reproduction

Subfield: Ecology, Evolution, Behavior and Systematics

Field: Agricultural and Biological Sciences

Domain: Life Sciences

Open Access status: gold

Estimated APC paid: $1,500

Funders: Wellcome Trust, Leverhulme Trust