Work

An optimized protocol for chromatin immunoprecipitation from murine inguinal white adipose tissue

Year: 2023

Type: article

Abstract: Chromatin immunoprecipitation (ChIP) protocols have been used to reveal protein-DNA interactions of various cell types and tissues; however, optimization is required for each specific type of sample. Here, we present a ChIP protocol from murine inguinal white adipose tissue. We describe steps for tissue harvesting, crosslinking, chromatin extraction, shearing, immunoprecipitation, and purification. We then detail procedures for analysis including library preparation, sequencing, and qRT-PCR validation. For complete details on the use and execution of this protocol, please refer to Antonia Katsouda et al. (2022).1

Source: STAR Protocols

DOI: 10.1016/j.xpro.2023.102594

Authors: Antonia Katsouda, Dimitrios Valakos, Giannis Vatsellas, Dimitris Thanos, Andreas Papapetropoulos

Institutions: National and Kapodistrian University of Athens, Biomedical Research Foundation of the Academy of Athens

Language: English

Topic: Genomics and Chromatin Dynamics

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $1,500