Work

Optimizing cryopreservation of nasal polyp tissue for cellular functional studies and single‐cell RNA sequencing

Year: 2023

Type: article

Abstract: KEY POINTS: Mast cell numbers were reduced in samples cryopreserved as whole tissue chunks. Thawed epithelial cells had reduced proliferation rates when preserved as dissociated cell suspensions. The right cryopreservation method to choose may depend on the goals and cell-type focus of the project.

Source: International Forum of Allergy & Rhinology

DOI: 10.1002/alr.23275

Authors: Aaqib Sohail, Carolyn H. Baloh, Jonathan Hacker, Laura Cho, Tessa Ryan, Regan W. Bergmark, Stella E. Lee, Alice Maxfield, Rachel Roditi, Daniel F. Dwyer, Kathleen M. Buchheit, Tanya M. Laidlaw

Institutions: Brigham and Women's Hospital, Harvard University

Language: English

Topic: Sinusitis and nasal conditions

Subfield: Otorhinolaryngology

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: green

Funder: National Institute of Allergy and Infectious Diseases

Awards: The Role of IL-5 and Local Nasal Polyp Immunoglobulin Production in Aspirin-Exacerbated Respiratory Disease, Pathophysiologic and Therapeutic Mechanisms of Aspirin Exacerbated Respiratory Disease, Patient-Oriented Research Mentorship and Training in Upper Airway Allergic and Inflammatory Diseases

OpenAlex ID: W4386993766

FWCI: 0.527

Cites: 7

Cited by: 2

Related to: 10