Work

An interaction network in the polymerase active site is a prerequisite for Watson-Crick base paring in Pol g

Year: 2023

Type: article

Abstract: Pol gamma is essential for mitochondrial genome integrity. Mutations to the polymerase causes disruption to it's various functions. Mutations in the 853 site causes various neurological issues. Structural and computational analysis of the wild-type and the mutants show that the mutation inhibits the process of opening and closing of the polymerase necessary for nucleotide incorporation. Furthermore, the dNTP does not form Watson-Crick base pairing with the templating residue, which is necessary for the reaction to take place. Hence, we see that the single point mutation causes changes to the polymerase that inhibits its functionality.

Source: Zenodo (CERN European Organization for Nuclear Research)

DOI: 10.5281/zenodo.8377288

Authors: Park, Joon, Herrmann, Geoffrey K., Roy, Arkanil, Shumate, Christie K., Cisneros, G. Andrés, Yin, Y. Whitney

Institutions: The University of Texas Medical Branch at Galveston, The University of Texas at Dallas

Language: English

Topic: DNA and Nucleic Acid Chemistry

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

OpenAlex ID: W4387032513

FWCI:

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