Year: 2020
Type: article
Abstract: Y-STRs are specialized class of short tandem repeats (STRs) located on human Y (male) chromosomes and are passed unchanged (barring a mutation) from one generation to the next.They are widely used in population genetic studies, forensics, Paternity and genealogical DNA testing and unknown male identification.A common Problem with this kind of analysis is the decomposition of DNA by bacteria and other microorganisms, the simultaneous exposure to environmental agents results in DNA degradation in postmortem tissues. Materials and Methods:The DNA extracted from the blood samples of the members of two families by(usingQIAamp® DNA Mini Kit (QIAGEN, USA), as well as from bone sample of the corpse by decalcifying steps , then followed by organic method for DNA extraction.STR amplification and typing:using an AmpFlSTR®Identifiler® plus PCRAmplification Kit (AppliedBiosystems).andAmpFlSTR®Yfiler® PCR Amplification Kit.We separate amplicons using a geneticanalyzer (ABI 3130; AppliedBiosystems).Then analyzed the amplicons with the suitable software (GeneMapperID, version 3.2; Applied Biosystems) using standard procedures. Results:The corpse was the son of the second family not the first one. Conclusion:In forensic genetics, applications Y-STRs are useful for discrimination of paternal linkage rather than for individual identification.The male specific part of the human Y chromosome is widely used in forensic DNA analysis, particularly in cases where standard autosomal DNA profiling is not informative.
Source: المجلة الجنائية القومية
Author: أماني موسي
Institution: October 6 University
Language: Arabic
Subfield: Genetics
Domain: Life Sciences
Open Access status: bronze
OpenAlex ID: W4387039445